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| Registros recuperados: 33 | |
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| Ronzani Cerqueira, Vinicius. |
| Several sea bass-rearing experiments were performed from hatching trough metamorphosis. The rearing units employed were 500 1 polyester tanks, where flow rate and temperature of seawater, light and suitable food (rotifer and artemia) were controlled. Stock density ranged between 50 and 100 larvae per liter. Individuals held in tanks with internal black walls developed faster and had a greater survival rate than those in white tanks. This suggests that a better contrast between preys and background assists the larvae to see food organisms more clearly. The sea bass larvae search for food is based only in vision and not trought mechanoreceptors or chemoreceptors. It was very difficult to evaluate the light intensity (50-5000 lux) influence on feeding,... |
| Tipo: Text |
Palavras-chave: Food; Prey; Light; Zootechnical; Digestive transit; Temperature; Dicentrarchus labrax; Intensive rearing; Sea bass. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/1986/these-1676.pdf |
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| Fauvel, Christian; Suquet, Marc; Dreanno, Catherine; Zonno, Vincenzo; Menu, Bruno. |
| A sperm cryopreservation protocol adapted from turbot, was tested on sea bass using either 250-mu L straws or 1.5-mL cryovials. A dilution to 1/3 in Mounib's extender and a cooling rate of 65 degrees C.min(-1) allowed frozen sperm to recover an initial motility similar to that of fresh sperm at thawing; however, significant differences in motility (P < 0.001, n = 10 fish semen) were observed at further post-activation times, the motility decrease being faster in thawed sperm. At the experimental scale, triplicate inseminations of 2-mL aliquots (approximately 2 000 eggs) showed a significant fertility decay of thawed sperm compared to that of fresh sperm (P < 0.01, n = 12 fish semen) when a discriminating 35.10(3) spermatozoa to egg ratio was applied.... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Cryobank; Insemination; Spermatozoa; Cryopreservation; Dicentrarchus labrax; Loup; Banque de sperme; Insémination; Cryoconservation; Cryoconservation. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-866.pdf |
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| Peruzzi, Stefano; Chatain, Beatrice. |
| The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0-1 degrees C for 15-20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm(-2)) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was... |
| Tipo: Text |
Palavras-chave: Parental variability; Pressure and cold shocks; Dicentrarchus labrax; Sea bass; Triploidy; Meiotic gynogenesis. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-507.pdf |
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| Pichavant, Karine; Person, Jeannine; Le Bayon, Nicolas; Severe, Armelle; Le Roux, Annick; Boeuf, Gilles. |
| When juvenile turbot Scophthulmus maximus and sea bass Dicentrarchus labrax were fed to satiation, growth and food intake were depressed under hypoxia (3.2 +/- 0.3 and 4.5 +/- 0.2 mg O-2 l(-1)). However, no significant difference in growth was observed between fishes maintained in hypoxia and fed to satiation and fishes reared in normoxia (7.4 +/- 0.3 mg O-2 l(-1)) and fed restricted rations (same food intake of fishes at 3.2 mg O-2 l(-1)). Routine oxygen consumption of fishes fed to satiation was higher in normoxia than in hypoxia due to the decrease in food intake in the latter. Of the physiological parameters measured, no significant changes were observed in the two species maintained in hypoxia. This study confirms the significant interaction between... |
| Tipo: Text |
Palavras-chave: Oxygen consumption; Growth; Hypoxia; Sea bass; Turbot. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-617.pdf |
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| Dosdat, Antoine; Person, Jeannine; Coves, Denis; Dutto, Gilbert; Gasset, Eric; Le Roux, Annick; Lemarie, Gilles. |
| The chronic effects of exposing sea bass (average initial weight 100 g) to ammonia in water at 22 degreesC were first evaluated over a 61-day period (period 1, P1) during which nine different groups were submitted to nine ambient ammonia levels ranging from 0.014 to 0.493 mg 1(-1) NH3-N (0.53-16.11 mg 1(-1) total ammonia nitrogen (TA-N)) and fed using self-feeders. At the end of P1, the fish were starved for 10 days (P2). Their recovery capacity was tested over 43 days (P3) after which the exogenous ammonia supply was stopped in all treatments and the fish were allowed to feed. After 20 days of exposure a highly significant effect of ammonia was evident from the decrease in feeding activity, voluntary feed intake (VFI) and specific growth rate (SGR), and... |
| Tipo: Text |
Palavras-chave: Sea bass; Self feeding; Compensatory growth; Chronic toxicity; Ammonia. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-595.pdf |
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| Morais, Sophia; Cahu, Chantal; Zambonino-infante, Jose-luis; Robin, Jean; Ronnestad, I; Dinis, M; Conceicao, L. |
| The influence of dietary TAG source (fish oil, triolein, and coconut oil) and level (7.5 and 15% of the diet) on growth, lipase activity, and mRNA level was studied in sea bass larvae, from mouth opening until day 24 and from day 37 to 52. Fish oil and triolein induced better growth in both experiments, this being significant at a higher dietary level. Coconut oil significantly decreased growth at the higher level, possibly as the result of an excessive supply of medium-chain TAG. Growth was not related to lipase specific activity, suggesting a production in excess to dietary needs. Body lipid content was positively related to dietary lipid level and was affected by lipid quality. In addition, larval FA composition generally reflected that of the diet. The... |
| Tipo: Text |
Palavras-chave: Lipase activity; Dietary; Growth; Larvae; Sea bass; Fish culture. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2004/publication-785.pdf |
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| Chatain, Beatrice; Poline, Olivier. |
| Objectives : Presently, most of marine fish farmers around the Mediterranean basin grow genetically wild fish strains, which have not been genetically improved for culture performance (growth, appearance, disease resistance etc.). It is inevitable that the present gap for genetically improved strains of marine fish, and their reliable supply, will be filled. It will ultimately dominate the competitive component of marine culture industries once they evolve into commodity-style agribusiness (with fish prices being set at the lowest possible cost of production and the best quality). Those countries, which can competitively develop and supply the improved strains, will enjoy the benefit (perhaps survival) for their local industries. They will avoid... |
| Tipo: Text |
Palavras-chave: Pathogen free; Genetic origin; Sperm; Cryopreservation; Sea bass. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/rapport-6494.pdf |
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| Sandblom, Erik; Farrell, Anthony; Altimiras, Jordi; Axelsson, Michael; Claireaux, Guy. |
| Cardiac preload (central venous; pressure, Pcv), mean circulatory filling pressure (MCFP), dorsal aortic blood pressure (P-DA) and relative cardiac output (Q) were measured in sea bass (Dicentrarchus labrax) at rest and while swimming at 1 and 2 BL s(-1). MCFP, an index of venous capacitance and the upstream venous pressure driving the return of venous blood to the heart, was measured as the plateau in Pcv during ventral aortic occlusion. Compared with resting values, swimming at 1 and 2BL s(-1) increased Q (by 15 +/- 1.5 and 38 +/- 6.5%, respectively), Pcv (from 0.11 +/- 0.01 kPa to 0.12 +/- 0.01 and 0.16 +/- 0.02 kPa, respectively), MCFP (from 0.27 +/- 0.02 kPa to 0.31 +/- 0.02 and 0.40 +/- 0.04 kPa, respectively) and the calculated pressure gradient for... |
| Tipo: Text |
Palavras-chave: Blood pressure; Cardiac rythm; Swimming aquaculture; Sea bass. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-421.pdf |
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| Peruzzi, Stefano; Varsamos, Stamatis; Chatain, Beatrice; Fauvel, Christian; Menu, Bruno; Falguiere, Jean-claude; Severe, Armelle; Flik, Gert. |
| The purpose of this study was to determine whether diploid and triploid sea bass differed in terms of main haematological and physiological characteristics. Diploid and triploid fish were produced by sub-optimal pressure treatments and held in communal environments under standard rearing conditions. Total red blood cell count (RBCC), haemoglobin concentration (Hb), hematocrit (Hct), mean cell volume (MCV), mean cellular haemoglobin content (MCH), mean cell haemoglobin concentration (MCHC), plasma metabolites, osmotic pressure, gill Na+/K+-ATPase activity, electrolytes, cortisol, and 3,5,3'-triiodo-L-thyronine (T-3) were measured and compared. Triploidisation in sea bass led to an increase in erythrocyte size (32% in cytoplasm surface area, and 50% in... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Triploid; Diploid; Physiology; Haematology. |
| Ano: 2005 |
URL: https://archimer.ifremer.fr/doc/2005/publication-508.pdf |
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| Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; Mckenzie, David; Bois, P. |
| The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage... |
| Tipo: Text |
Palavras-chave: Fatty acid; Sea bass; Ventricular myocyte; L type calcium channel; Oleic acid. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2120.pdf |
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| Coves, Denis; Beauchaud, M; Attia, J; Dutto, Gilbert; Bouchut, C; Begout, Marie-laure. |
| In two experiments, a computerized on-demand feeding system coupled with a PIT tag monitoring device was used to continuously record the triggering activity by ca. 50 individual sea bass for 55 days (Exp. 1, initial average body weight and coefficient of variation, CV: 299 g, 15%) and 69 days (Exp. 2, 157 g, 13%). Each group was stocked in 1 m(3) tanks and exposed to a water temperature of 21 +/- 1 degrees C and a light regime of 16:8 LD. Only 5% of the triggering activity was not associated with simultaneous PIT tag detection. Although each individual was detected via PIT tag during the experiment, only 67% and 74% of the fish actuated the trigger at least once in Exp. 1 and Exp. 2, respectively. Moreover, only two fish in Exp. 1 and one fish in Exp. 2... |
| Tipo: Text |
Palavras-chave: PIT tag detection antenna; Individual triggering activity; Self feeding; Sea bass. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1341.pdf |
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| Boutet, Isabelle; Ky, Chin Long; Bonhomme, Francois. |
| Euryhaline teleosts possess the capacity to osmoregulate under various environmental conditions (freshwater to hypersaline water). This physiological capacity is generally monitored using enzyme activity assays (Na+/K+-ATPase...), hormones quantification (prolactine, growth hormone) or their mRNAs expression. To date, few studies addressed the genetic correlates of adaptation to varying salinity at a molecular level in such fish. In the sea bass Dicentrarchus labrax, genetic differentiation was observed at specific allozyme loci between lagoon- and open-sea populations. In the present study, we investigated transcriptomic response of D. labrax to salt- and freshwater acclimation in two organs involved in osmoregulation, gill and intestine. By using... |
| Tipo: Text |
Palavras-chave: Suppression subtractive hybridisation; Carbonic anhydrase; Osmoregulation; MRNA expression; Sea bass. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1901.pdf |
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| Ky, Chin Long; De Lorgeril, Julien; Hirtz, C; Sommerer, N; Rossignol, M; Bonhomme, F. |
| The European sea bass, Dicentrarchus labrax L., tolerates a range of salinities from freshwater to hyper-saline. To study differences in protein expression, fish were reared in both freshwater and seawater. After 3-month acclimation, gill and intestine epithelia were collected and the soluble protein extracted. In all, 362 spots were differentially expressed in the gills and intestines of fishes reared in seawater compared to those from freshwater. Fifty differential protein spots were excised from a colloidal Coomassie-stained gel. Nine separate protein spots were identified unambiguously by mass spectrometry and database searching. Among the six proteins over-expressed in gill cells in seawater, five were cytoskeleton proteins and one was the aromatase... |
| Tipo: Text |
Palavras-chave: Two dimensional gel electrophoresis; Sea bass; Salinity; Real time PCR; MALDI TOF mass spectrometry. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3952.pdf |
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| Peruzzi, Stefano; Westgaard, Jon-ivar; Chatain, Beatrice. |
| Although the actiology of swimbladder inflation anomalies in important aquaculture species such as European sea bass D. labrax is not fully determined, culture conditions are commonly suggested as main contributory factors. Little information is available on whether swimbladder inflation has a genetic basis for its expression too. In this work, 24 full-sibling sea bass families from a 4 dams x 6 sires factorial crossing were reared under communal conditions. The larvae developing normal and abnormal (uninflated or hyper-inflated) swimbladders were genotyped at four microsatellite loci, Labrax-3, Labrax-13, Labrax-17, Labrax29, and allocated to the individual breeders. Out of 273 offspring, 97% could be assigned to a single parental pair. The genotype and... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; Sea bass; Genetics; Anomalies; Swimbladder. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2590.pdf |
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| Registros recuperados: 33 | |
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